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Rat NEFL (Neurofilament Light Polypeptide) ELISA Kit

Rat NEFL (Neurofilament Light Polypeptide) ELISA Kit

The Rat (NEFL) Neurofilament Light Polypeptide ELISA Kit measures Neurofilament Light Polypeptide in Rat samples. The plate has been pre-coated with Rat NEFL antibody. NEFL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NEFL Antibody is added and binds to NEFL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEFL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NEFL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1747Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.67 ng/L
Detection Range 1-400 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Neuroscience
Background Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Neurofilament Light Polypeptide
Regulatory Status For Research Use Only
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