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Rat NES-1 (Nesfatin 1) ELISA Kit
Rat NES-1 (Nesfatin 1) ELISA Kit
The Rat (NES-1) Nesfatin 1 ELISA Kit measures Nesfatin 1 in Rat samples. The plate has been pre-coated with Rat NUCB2 antibody. NUCB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NUCB2 Antibody is added and binds to NUCB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NUCB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NUCB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0878Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
16.23 ng/L
Detection Range
30-9000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (PubMed: 21653697). [Nesfatin-1]: Anorexigenic Peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Nesfatin 1
Regulatory Status
For Research Use Only
