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Rat NFKBIA (Nf-Kappa-B Inhibitor Alpha) ELISA Kit

Rat NFKBIA (Nf-Kappa-B Inhibitor Alpha) ELISA Kit

The Rat (NFKBIA) Nf-Kappa-B Inhibitor Alpha ELISA Kit measures Nf-Kappa-B Inhibitor Alpha in Rat samples. The plate has been pre-coated with Rat NFKBIA antibody. NFKBIA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NFKBIA Antibody is added and binds to NFKBIA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NFKBIA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NFKBIA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1817Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 5.25 ng/L
Detection Range 10-2000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Nf-Kappa-B Inhibitor Alpha
Regulatory Status For Research Use Only
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