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Rat ON (Osteonectin) ELISA Kit
Rat ON (Osteonectin) ELISA Kit
The Rat (ON) Osteonectin ELISA Kit measures Osteonectin in Rat samples. The plate has been pre-coated with Rat SPP1 antibody. SPP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SPP1 Antibody is added and binds to SPP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SPP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0278Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.54 ng/mL
Detection Range
1-400 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism
Background
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Osteonectin
Regulatory Status
For Research Use Only
