Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
The Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat PACAP-27. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
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