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Rat Pro-IL1B (Pro Interleukin 1 Beta) ELISA Kit

Rat Pro-IL1B (Pro Interleukin 1 Beta) ELISA Kit

The Rat (Pro-IL1B) Pro Interleukin 1 Beta ELISA Kit measures pro interleukin 1 beta in Rat samples. The plate has been pre-coated with Rat pro-IL1B antibody. pro-IL1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat pro-IL1B Antibody is added and binds to pro-IL1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated pro-IL1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat pro-IL1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2206Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 14.15 ng/L
Detection Range 30-9000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cytokines, Inflammation, Immunology
Background Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte pro interleukin 1 beta
Regulatory Status For Research Use Only
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