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Rat RAB3A (Ras-related Protein Rab-3A) ELISA Kit
Rat RAB3A (Ras-related Protein Rab-3A) ELISA Kit
The Rat (RAB3A) Ras-related Protein Rab-3A ELISA Kit measures Ras-related protein Rab-3A in Rat samples. The plate has been pre-coated with Rat RAB3A antibody. RAB3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat RAB3A Antibody is added and binds to RAB3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RAB3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat RAB3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2440Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
4.88 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (PubMed:21689256).
Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (PubMed:18407218).
Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (PubMed:17149709).
Stimulates insulin secretion through interaction with RIMS2 and RPH3AL effectors in pancreatic beta cells (By similarity).
Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (By similarity).
Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (By similarity).
Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (PubMed:28057568).
Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Ras-related protein Rab-3A
Regulatory Status
For Research Use Only
