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Rat S100A4 (S100 Calcium Binding Protein A4) ELISA Kit
Rat S100A4 (S100 Calcium Binding Protein A4) ELISA Kit
The Rat (S100A4) S100 Calcium Binding Protein A4 ELISA Kit measures S100 Calcium Binding Protein A4 in Rat samples. The plate has been pre-coated with Rat S100A4 antibody. S100A4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A4 Antibody is added and binds to S100A4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated S100A4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat S100A4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1341Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
5.63 ng/L
Detection Range
10-4000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (By similarity). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (By similarity). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
S100 Calcium Binding Protein A4
Regulatory Status
For Research Use Only
