The Rat (SLC47A1) Multidrug and Toxin Extrusion Protein 1 ELISA Kit measures Multidrug and Toxin Extrusion Protein 1 in Rat samples. The plate has been pre-coated with Rat SLC47A1 antibody. SLC47A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SLC47A1 Antibody is added and binds to SLC47A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC47A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SLC47A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundSolute transporter for tetraethylammonium (TEA), cimetidine, metformin, guanidine, N-methylnicotinamide (NMN) and also the zwitterionic cephalosporin cephalexin. Not a transporter for 1-methyl-4-phenylpyridinium (MPP), procainamide, creatinine, guanidine, p-aminohippurate (PAH) and the anionic cephalosporin cefalozin. MPP-transport activity has been observed in PubMed: 16928787 and PubMed: 17047166 and may contradict results observed in PubMed: 16850272. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Responsible for the secretion of cationic drugs across the brush border membranes. Source: UniProt Consortium (2025)
