Rat TNFSF13 (Tumor necrosis factor ligand superfamily member 13) ELISA Kit
Rat TNFSF13 (Tumor necrosis factor ligand superfamily member 13) ELISA Kit
The Rat TNFSF13 (Tumor necrosis factor ligand superfamily member 13) ELISA Kit measures TNFSF13/APRIL in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNFSF13/APRIL antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNFSF13/APRIL bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNFSF13/APRIL in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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