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Rat TPI1 (Triosephosphate Isomerase) ELISA Kit
Rat TPI1 (Triosephosphate Isomerase) ELISA Kit
The Rat (TPI1) Triosephosphate Isomerase ELISA Kit measures Triosephosphate isomerase in Rat samples. The plate has been pre-coated with Rat TPI1 antibody. TPI1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TPI1 Antibody is added and binds to TPI1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TPI1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TPI1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2298Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
9.95 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.
It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Triosephosphate isomerase
Regulatory Status
For Research Use Only
