1
/
of
1
Rat TRIB3 (Tribbles Homolog 3) ELISA Kit
Rat TRIB3 (Tribbles Homolog 3) ELISA Kit
The Rat (TRIB3) Tribbles Homolog 3 ELISA Kit measures Tribbles homolog 3 in Rat samples. The plate has been pre-coated with Rat TRIB3 antibody. TRIB3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRIB3 Antibody is added and binds to TRIB3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIB3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TRIB3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2296Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Rat
Sensitivity
9.17 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (By similarity).
Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (By similarity).
Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity).
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (PubMed:15469416).
May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity).
Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases (By similarity).
Can inhibit APOBEC3A editing of nuclear DNA (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Tribbles homolog 3
Regulatory Status
For Research Use Only
