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Rat TRIM72 (Tripartite Motif-containing Protein 72) ELISA Kit

Rat TRIM72 (Tripartite Motif-containing Protein 72) ELISA Kit

The Rat (TRIM72) Tripartite Motif-containing Protein 72 ELISA Kit measures Tripartite motif-containing protein 72 in Rat samples. The plate has been pre-coated with Rat TRIM72 antibody. TRIM72 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRIM72 Antibody is added and binds to TRIM72 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIM72 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TRIM72. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2301Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 10.44 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Tripartite motif-containing protein 72
Regulatory Status For Research Use Only
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