The Rat (TRPA1) Transient Receptor Potential Cation Channel Subfamily A Member 1 ELISA Kit measures Transient receptor potential cation Channel subfamily A member 1 in Rat samples. The plate has been pre-coated with Rat TRPA1 antibody. TRPA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRPA1 Antibody is added and binds to TRPA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRPA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TRPA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundReceptor-activated non-selective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Shows 8-fold preference for divalent over monovalent cations. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of irritants, such as allylthiocyanate (AITC) found in mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta9-tetrahydrocannabinol (THC), the psychoactive component of marijuana (PubMed:14712238).
Is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteTransient receptor potential cation Channel subfamily A member 1
