The Rat (TRPV4) Transient Receptor Potential Cation Channel Subfamily V Member 4 ELISA Kit measures Transient receptor potential cation Channel subfamily V member 4 in Rat samples. The plate has been pre-coated with Rat TRPV4 antibody. TRPV4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRPV4 Antibody is added and binds to TRPV4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRPV4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TRPV4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundNon-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11081638).
Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:11081638).
Also activated by heat, low pH, citrate and phorbol esters (By similarity).
Increase of intracellular Ca2+ potentiates currents (By similarity).
Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity).
Acts as a regulator of intracellular Ca2+ in synoviocytes (By similarity).
Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity).
Together with PKD2, forms mechano- and thermosensitive channels in cilium (By similarity).
Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity).
Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity).
Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity).
Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity).
Required for normal development and maintenance of bone and cartilage (By similarity).
In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteTransient receptor potential cation Channel subfamily V member 4
