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Rat TRX (Thioredoxin) ELISA Kit
Rat TRX (Thioredoxin) ELISA Kit
The Rat (TRX) Thioredoxin ELISA Kit measures Thioredoxin in Rat samples. The plate has been pre-coated with Rat TXN antibody. TXN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TXN Antibody is added and binds to TXN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TXN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TXN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0444Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.53 ng/mL
Detection Range
1-400 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism
Background
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Thioredoxin
Regulatory Status
For Research Use Only
