The Rat (UBIAD1) Ubia Prenyltransferase Domain-containing Protein 1 ELISA Kit measures UbiA prenyltransferase domain-containing protein 1 in Rat samples. The plate has been pre-coated with Rat UBIAD1 antibody. UBIAD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat UBIAD1 Antibody is added and binds to UBIAD1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated UBIAD1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat UBIAD1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPrenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays an important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteUbiA prenyltransferase domain-containing protein 1
