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Recombinant Human VDB (C-6His)
Recombinant Human VDB (C-6His)
Recombinant Human Vitamin D-Binding Protein is produced by our Mammalian expression system and the target gene encoding Leu17-Leu474 is expressed with a 6His tag at the C-terminus.
Catalog No:
EPT229
Regular price
$199.00 USD
Regular price
Sale price
$199.00 USD
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per
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Product Details
Accession
P02774
Molecular Weight
52.3 KDa
Apparent Molecular Weight
53 KDa, reducing conditions
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Expression Host
Mammalian
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping Condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed in the Storage field.
Storage Condition and Shelf Life
Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.
Background
Vitamin D-Binding Protein (DBP) is a member of the ALB/AFP/VDB family. DBP is a secreted protein and contains three albumin domains. The primary structure contains 28 cysteine residues forming multiple disulfide bonds. DBP acts as a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. DBP binds to vitamin D and its plasma metabolites and transports them to target tissues. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
Analyte
VDB
Regulatory Status
For Research Use Only
