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Sheep SDH (Sorbitol Dehydrogenase) ELISA Kit

Sheep SDH (Sorbitol Dehydrogenase) ELISA Kit

The Sheep (SDH) Sorbitol Dehydrogenase ELISA Kit measures Sorbitol Dehydrogenase in Sheep samples. The plate has been pre-coated with Sheep SDH antibody. SDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep SDH Antibody is added and binds to SDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep SDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0143Sh
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Sheep
Sensitivity 0.098 ng/mL
Detection Range 0.2-60 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction, Metabolism
Background Polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols. Is mostly active with xylitol, L-iditol and D-sorbitol (D-glucitol) as substrates, leading to the C2-oxidized products D-xylulose, L-sorbose and D-fructose, respectively (PubMed:1459146). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Sorbitol Dehydrogenase
Regulatory Status For Research Use Only
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