T-SOD (Total superoxide dismutase) ELISA Kit
T-SOD (Total superoxide dismutase) ELISA Kit
The T-SOD (Total superoxide dismutase) ELISA Kit measures T-SOD in Human, Cynmolgus, Rhesus monkey, Goat, Duck, Hamster, Porcine, Rat, Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti T-SOD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with T-SOD bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of T-SOD in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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