• IP antibody use：5ug V5 Mouse IgG1 per ml Lysate, WB 1:5000 1. untransfected 293 cell lysate 2. transfected 293 cell lysate with V5-tag fusion protein 3. IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4. IP (transfected 293+ normal Mouse IgG+Protein G ag
  • IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
  • 1ug V5 fusion protein+ Primary antibody dilution at 1. 1:5,000 2. 1:10,000
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V5-Tag Mouse mAb

V5-Tag Mouse mAb

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.

Catalog No: EM1004
$148.00 USD
$148.00 USD
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Packaging
1.5 weeks
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Product Details

Application(s) WB, IP, IF
Source Murine
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1 mg/mL
Dilution WB 1:5,000 IP 1:200 IF 1:1,000
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20C. Avoid repeated freeze-thaw cycles.
Analyte V5-Tag
Regulatory Status For Research Use Only
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