Zebrafish IL-1b (Interleukin 1 Beta) ELISA Kit
Zebrafish IL-1b (Interleukin 1 Beta) ELISA Kit
The Zebrafish IL-1b (Interleukin 1 Beta) ELISA Kit measures IL-1Beta in Zebrafish Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with IL-1b. IL-1b in the sample or standard competes with a fixed amount of biotin-labeled IL-1b for sites on a pre-coated antibody specific to IL-1b. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of IL-1b in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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